| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ritchie, Matthew J (9)
|
UNAT |
35.45 |
36.14 |
0.69 |
| 2 |
Delos Santos, Joshua B (8)
|
NCGFSC |
39.59 |
37.19 |
-2.40 |
| 3 |
Garrett, Logan F (10)
|
NCYBAC |
41.36 |
41.03 |
-0.33 |
| 4 |
Rabon, Matthew R (10)
|
NCGFSC |
43.55 |
42.61 |
-0.94 |
| 5 |
Clayton, Thomas J (9)
|
NCGFSC |
38.97 |
45.48 |
6.51 |
| 6 |
Bacon, Ty M (10)
|
NCGFSC |
43.70 |
45.79 |
2.09 |
| 7 |
Earl, Tyler K (10)
|
UNAT |
49.27 |
45.84 |
-3.43 |
| 8 |
Johnson, Connor R (10)
|
NCGOLD |
52.14 |
48.01 |
-4.13 |
| 9 |
Pendleton, Zackary A (10)
|
NCGFSC |
59.59 |
54.42 |
-5.17 |
| 10 |
Jones, Noah C (9)
|
NCYBAC |
48.07 |
56.28 |
8.21 |
| 11 |
Wozniak, Dominic I (9)
|
NCRMY |
|
57.50 |
- |
| 12 |
Rodeffer, Matthew J (9)
|
NCLTNC |
|
1:02.91 |
- |
| 13 |
Price, Ethan R (10)
|
NCRMY |
|
1:10.53 |
- |